What is decalcify bone?

Decalcification refers to the removal of calcium from a bone or tissue sample, usually done for histological or pathological analysis. When bones undergo decalcification, they become softer and more pliable, which makes it easier to section the bone for microscopic analysis. The process usually involves the use of a decalcifying agent, such as ethylene diamine tetra-acetic acid (EDTA) or nitric acid, which dissolves the calcium in the bone. After decalcification, the bone can be stained and examined under a microscope to identify any abnormalities such as fractures, tumors, or other pathological conditions.